Fusarium head blight (Fusarium graminearum, Schwabe; FHB) is a devastating disease affecting all classes of wheat (Triticum aestivum L.) crops. A field experiment with spring wheat cv. AC Brio including five N application treatments and three planting dates was conducted for two years at Ottawa, ON. The objective of the study was to assess how management practices affect incidence and severity of FHB, and if harvesting time affects the accumulation of deoxynivalenol (DON) in the grain. From physiological maturity, within the optimum planting plots, weekly samples were taken for the determination of DON in the grain. Our data showed that late planting resulted in the highest DON concentration, and delaying harvest after physiological maturity increased significantly DON concentration in the grain. This study indicates that timely planting and harvesting may serve as practical measures to reduce DON concentration in wheat grain.