Application of animal manure to meet the nitrogen (N) needs of crops can lead to accumulation of macro- and micro-nutrients in the soil. The objectives of this study were to determine the accumulation, depth distribution and bioavailability of copper (Cu) and zinc (Zn) in soils amended with liquid dairy manure (DM) and solid poultry layer litter (PL) for up to 40 yr. Current application rates and manure composition suggested annual additions of 167 - 188 kg P ha^-1, 8.2 – 9.2 kg Cu ha^-1 and 4.8 – 5.4 kg Zn ha^-1 for dairy manure, and 206 - 240 kg P ha^-1, 1.2 – 1.4 kg Cu ha^-1 and 7.1 – 8.3 kg Zn ha^-1 for poultry litter. Soil total Cu and Zn contents were positively correlated with total P with more rapid Zn accumulation in the plow layer (0-15 cm) in PL fields and greater Cu accumulation in the DM fields. Accumulation of Cu and Zn was limited to the plow layer only in all but one field analyzed. In the PL field with the longest manure history, soil Zn levels were elevated to a depth of 30 cm. Leachate from intact columns showed very low Cu and Zn concentrations and 0.01 M CaCl₂ extractions of plow layer samples showed no risk of toxicity to most field crops. Implementation of the P runoff index will discontinue manure application on high P fields and thus prevent further buildup of Cu and Zn to concentrations of environmental concern.