Annual pine straw harvest offers additional income for small landowners.� However, this practice disrupts nutrient cycling and fertilizer amendment is needed to offset depletion in surface soil fertility.� A plot study comparing straw harvest without fertilization, with inorganic fertilization and organic (poultry litter) fertilization to control was established in 2002 on a Ruston (fine-loamy, siliceous, thermic Typic Paleudult) soil.� Baseline and subsequently annual surface (0 � 15 cm) soil samples were collected prior to fertilizer application.� Runoff plots (1 m x 1 m) were installed in replicate plots and runoff from simulated rainfall collected prior to fertilizer application.� Soil samples indicate that chemical fertility has been adequately maintained by replenishment with inorganic and poultry litter fertilizer, however, with increase in soil P, particularly where poultry litter was used.� Losses of P in runoff were variable within treatments but generally paralleled increased soil P concentrations.� Approximately 80 % of the P in runoff was molybdate- reactive.� Use of simple Langmuir isotherms for P desorption into runoff adequately described P losses in most cases.