Ammonia volatilization can be a major pathway of nitrogen (N) loss from turfgrass systems. This study was conducted to determine if mowing had an effect on N loss via ammonia (NH₃) volatilization from six fertilizers applied to turfgrass. The effects of mowing on NH₃ volatilization were studied in Auburn, AL, at the Turfgrass Research Unit on a bentgrass (Agrostis stolonifera L. var. palustris (Huds.) Farw.) putting green. Six N sources were used in the study: Urea (46% N), Sulfur Coated Urea (SCU) (39% N), Polyon (41% N), Methex (40% N), Ammonium Nitrate (34% N), and Milorganite (6% N). There was never an interaction between mowing N source with regard to NH₃ volatilization. Over the ten day measurement period, urea had the greatest NH₃ volatilization, with the highest proportion of that being released in the first two days after treatment. Polyon released the least NH₃ with the greatest release being nine and ten days after treatment. By the end of 10 days, mean volatilization loss of urea and SCU were not different. Polyon, Methex, ammonium nitrate, and Milorganite did not differ in NH₃ volatilized at the end of 10 days. Treatments in order from most to least NH₃ volatilization were: urea, SCU, Methex, ammonium nitrate, Milorganite, and Polyon. Mowing did not have an effect on the amount of N lost to NH₃ volatilization in these fertilizers applied to turfgrass. However, different N sources do have different rates of NH₃ volatilization over time.