There are three objectives for this study: to determine the within-row plant spacing and time of planting that will produce optimal yields and seed isoflavone content, to explore the feasiblity of incorporating edamame soybeans in a double-cropping system with strawberries, and to study the potential as an edamame soybean of newly identified line TN03-349. TN03-349 was planted into black plastic, irrigated strawberry beds in an East Tennessee location at five different within-row spacings (7.62,15.24,30.48,60.96,and 121.92 cm) in 2004 and 2005. Another strawberry bed planting was located in Middle Tennessee in 2005. Four soybean lines and two planting dates were used in the Middle Tennessee experiment. Two lines are high yielding soybean checks, while the third is a commercially available edamame cultivar. The fourth line is TN03-349. Planting dates were May 24 and June 14, 2005. A final field experiment utilized the same four soybean lines and planting dates with an additional planting on July 6, 2005. Four different within-row spacings were used as well. All experimental plantings were harvested at both the R6 (green) and R8 (dry) stages. Preliminary data indicates that isoflavone content was not affected by within-row plant spacing in the 2004 East Tennessee strawberry bed experiment. Yield data from the same experiment seems to indicate that soybeans were able to compensate for fewer plants per row at the 7.62,15.24,and 30.48 cm spacings. Yield dropped sharply at the 60.96 and 121.92 cm treatments. Line TN03-349 produced beans with large seed size and nutty flavor, traits which are essential for edamame soybeans.