Soybean cultivars (Glycine max [L.] Merr.) have been differentiated into two distinct genotype groups based on their ability to translocate chloride. In this project, the goal will be to evaluate how these genotype groups respond to high chloride irrigation water applied for several seasons to a field with no history of high salt content. An irrigation well was selected that had been recently identified as having elevated chloride levels. A randomized complete block test with ten replications was designed to measure the cumulative effects of high chloride irrigation water over a period of several growing seasons on three chloride-includer and three chloride-excluder cultivars. Water samples were taken from the well outlet during each irrigation. Leaflets were collected at each irrigation and seed was harvested after all cultivars had matured. Following each irrigation in the 2002, 2003, and 2005 growing season, includer cultivars had higher concentrations of chloride in their leaf tissues than did excluder cultivars (Fig.2). The amount of chloride detected in includer leaf tissues also increased after each irrigation, while the levels in the excluder cultivars remained about the same.