Fatty acid (FA) composition of forages has been shown to affect animal tissue and milk fatty acid composition. Leaf FA composition has also been related to cold and freeze tolerance in forage crops. Bahiagrass FA composition as a percent of total extracted FA's (%TEFA) was quantified to determining genotype diversity. Nine vegetative clones composed of one tetraploid (4n) and eight diploid (2n) and one population ('Tifton 9') were sampled during the fall of 2005. Seven FA's accounted for the largest portion of genotype %TEFA (92.0-94.4). Myristic (C14:0) and oleic (C18:1)%TEFA did not differ across 4n 'Argentine' and seven (2n) clones. Five FA's (C:16:0, C18:0, C:16:1, C18:2, C18:3)%TEFA varied according to genotype. Linolenic (C18:3) accounted for the major %TEFA in clones(47.4-54.4)followed by palmitic (C16:0) and linoleic (C:18:2). Results showed small differences in fatty acids between genotypes grown under the same conditions other than for C18:3 and C18:2. 'Argentine' had the highest C18:3 %TEFA, which prevents incorporating this trait through traditional breeding methods due to apomixis.