Soil management and landscape factors influence greenhouse gas emissions interactively rather than in isolation. Evaluation of effects of these factors and their interactions on greenhouse gas emissions is necessary for site specific management. This study evaluates effect of tillage, dairy manure application, and landscape position on nitrous oxide (N₂O), methane (CH₄) and carbon dioxide (CO₂) emissions from a corn/cotton rotation farming system. Gas samples were collected using a closed chamber method near Shorter, AL in spring 2004. Treatments included no-till (NT) and conventional tillage (CT) with or without dairy manure application distributed over three landscape positions: concave, sideslope, and upland. Dairy manure increased CH₄ emission while tillage decreased emissions. Concave positions recorded the highest CH₄ emissions under both tillage systems while upland positions consumed CH₄ (where no manure was applied). Tillage increased CO₂ emission. Dairy manure decreased CO₂ emission on sideslopes by 9% under CT, but increased emissions by 20% under NT. Tillage increased N₂O emission on all landscape positions with the increase being more pronounced on concave slopes. Dairy manure increased N₂O emissions on concave zones under both tillage systems. Treatments on concave positions generally had higher emission of the three gases, and greater soil moisture in treatments without dairy manure application. Similar CO₂ and N₂O emissions were observed in summer and fall seasons, but during winter CT significantly decreased CO₂ emission compared to NT. Adopting no-till in spring through fall can decrease CO₂ and N₂O emissions in agricultural systems. However, conventional tillage reduces CH₄ emissions. Dairy manure application may convert upland landscapes from being CH₄ sinks to CH₄ sources. It is apparent that soil management and landscape position interact to control greenhouse gas emissions from agricultural fields.