Assessment of soil C is important for determining the effects of Everglades restoration projects on C cycling and transformations. Accurate measurement of soil total C by automated CNS analysis may be confounded by the presence of CaCO3 in Everglades wetlands. The objectives of this study were to compare a loss-on-ignition (LOI) method with CNS analysis for assessment of soil C across a diverse group of calcareous Everglades wetlands. Over 3168 samples were taken for three soil depths (floc, 0-10, 10-30 cm) in 14 wetlands and analyzed for LOI, total C, and total Ca. The LOI method compared favorably to CNS analysis for LOI contents ranging from 0-1000 g kg-1, and for soil total Ca levels from 0-500 g Ca kg-1. For all wetlands and soil depths, LOI was significantly related to total C (r2=0.957). However, LOI was a better predictor of total C when LOI exceeded 400 g kg-1 due to less interference by CaCO3. Total C measurement by CNS analysis was problematic in soils with high total Ca and low LOI. Below 400 g kg-1, the presence of CaCO3 confounded C analysis. Inclusion of total Ca in regression models with LOI significantly improved the prediction of total C. Estimates of total organic C by CNS analysis were obtained by accounting for C associated with CaCO3 by calculation, with results being similar to total organic C values obtained from LOI analysis. The proportion of C in organic matter measured by the LOI method (51%) was accurate and applicable across wetlands, soil depths, and total Ca levels, thus LOI was a suitable indicator of total organic C in Everglades wetlands.