In Europe, the leveling of slopes almost always precedes new plantations of vineyards on hill. This work involves the movement and redistribution of large quantities of soil. Deep ploughing of the field surface follows land-leveling operations. All these operations increase hydrological variability of soil on slope and support the diffusion of precision farming. Currently, the cost needed to set up precision agriculture can be only supported by farms producing highly rentable goods. Increased yield and quality of the foods are the key factors which can lead to the popularization of the technique. Aim of this work was to compare different methods to map the hydropedological variability of vineyards and to assess its relevance for grape yield and wine quality. Two specialized vineyards (2 ha each) were investigate at Cetona (central Italy), in similar geomorphological settings. Hydropedological properties of soils at summit, backslope and footslope were characterized by profiles, auger-holes and mini-pits, as well as with a 4 year monitoring of soil water content, redox potential, and temperature. In 2006 John Deere Europe carried out a detailed soil, geophysical and topographic survey, measuring electromagnetic conductivity and resistivity, penetrometry and friction. A set of 47 thematic maps were produced, providing information about topography, flow direction, rooting depth, soil texture, water content and available water capacity at different depth. The maps “field capacity” and “permanent wilting point” of the root zone were used for a subdivision of the two vineyards in three sub areas. The resulting six zones were monitored in 12 plots for vine growing, grape yield and wine quality. The results demonstrated that response of Sangiovese vine was influenced by both soil type and topographic position. The sub area subdivision was functional in one of the two vineyards, the inaccuracies were due to flaws in soil functional parameter estimation and spatialization.