A large number of sediment samples from canals and wetlands in the middle Everglades of Florida were collected with a piston corer sediment sampler, separated the intact core column into different layers, and chemically analyzed. Geographic Information System (GIS) was used to analyze and process the data. The results showed that total phosphorus from canal sediments were extremely high compared to wetland, and a higher proportion of phosphorus in wetland sediments is more “active” than that in the canals mainly attributed to vegetation recycling.