Nitrogen is the most limiting factor for crop growth, fruiting, lint yield and quality in cotton. A study was conducted to determine the relationships between leaf nitrogen, cotton growth, and lint yield of four transgenic cotton cultivars (DP 555B/R, FM 960B/R, SG 215BG/RR and ST 559B/R) across four N fertilizer rates on a Leeper silt clay loam at Mississippi State (33°28'N, 88°47'W), MS. The treatments were: (1) no N applied during the growing season; (2) 56 kg N ha-1 applied at the second true leaf stage; (3) 112 kg N ha-1, equally split and applied at the second true leaf stage and at the first square stage; and (4) 168 kg N ha-1 split into two applications of 56 and 112 kg N ha-1 and applied at the second true leaf stage and at the first square stage, respectively. Plant height, mainstem node number, leaf area, above-ground plant component dry weights, and N were measured at specific growth stages. Dates of squaring, flowering and boll opening were also recorded. At the end of the season, lint yield and fiber quality were also measured. In addition, 1-m row of plants were mapped to understand cultivar and N interaction on boll retention and weight distribution patterns. Lint yield increased with higher rates of nitrogen fertilizer across all cultivars; the response was cultivar and weather dependent. Higher yields were associated with greater early-season growth. Boll distribution patterns varied among cultivars and nitrogen fertilizer rates. Low nitrogen treatments produced fewer bolls in the 2nd and 3rd positions and reached cut-out much earlier than the other treatments. The DP 555 BG/RR had longer fruiting period and produced more bolls at higher positions on the plant resulting in greater yields across N treatments in both the years.