The development an efficient and accurate method to predict in-season N requirements for winter wheat in the southeast is needed given the economic and environmental concerns surrounding N fertilizer use. The Amino Sugar Nitrogen Test (ASNT) which determines a potentially mineralizable soil N fraction shows promise for making in season N predictions. The objective of this research is to develop an in-season fertilizer N recommendation system using ANST for winter wheat in the humid southeast. Field experiments were conducted under conventional and no-till conditions during the 2005-2006 and 2006-2007 growing season at 28 sites across North Carolina. A randomized complete block design with 5 replications was used with N treatment as the main plot factor. There were 7 fertilizer N treatments ranging from to 0 to 168 kg ha-1. Soil samples were collected at planting and GS25 to a depth of 30 cm and analyzed for nitrate ,ammonium and amino sugar N (ASN). ASN analysis was conducted using a modification of the procedure described by Khan et. al (2001). The fertilizer N treatments were applied at GS-30. At harvest grain and straw N analysis were conducted on each treatment plot in order to ascertain crop N uptake. Native soil N uptake was determined from the unfertilized check plots and was modeled using soil ASN and residual soil nitrate and ammonium as indicator variables.This research shows that native soil N uptake by a wheat crop over the growing season may be predicted using the ASNT and a soil nitrate and ammonium test. This shows the potential for these tests to accurately predict optimum fertilizer N rates for wheat and further study is continuing to explore these possibilities.