Many low-molecular mass organic acid (LMMOA) anions are known to exist in soils and are suspected to strongly impact rhizosphere processes. One such LMMOA, 2-ketogluconate (kG), exists in rhizosphere soils as a byproduct of microbial activity and is suspected to play a key role in increasing soil nutrient availability. Previous studies suggest that kG is specifically (strongly) retained by the soil minerals gibbsite, goethite and kaolinite. Ketogluconate adsorption is dependent on pH, but independent of ionic strength. Moreover, these studies show that kG competes with phosphate, arsenate, and sulfate for adsorption sites. The objectives of this study were to determine the influence of kG on the surface chemistry of goethite and gibbsite, and to examine the influence of temperature on the adsorption of kG. Continuous potentiometric titrations were performed to assess the influence of kG on mineral surface potentials. Moreover, a thermodynamic assessment was performed by developing batch adsorption isotherms as a function of temperature.