For 95 years the Knorr-Holden Plots near Mitchell, Nebraska have had been in a continuous corn (Zea mays L.) cropping system. Studies of this duration are rare due to the difficulty of maintaining plots over extended periods of time. When studies do survive for nearly a century they provide unique opportunities to study the effects of management practices on crops and soils. The original plot, initiated in 1912, involved continuous corn with no fertilizer or manure applied. In 1942 this original plot was split into two plots; one plot began receiving an annual application of manure (27 Mg ha-1) while the other plot continued to receive nothing. In 1942 a second replication of these two treatments was added. In 1953 each manured and nonmanured plot was subdivide into six plots. These plots began receiving annual applications of six fertilizer treatments (0, 45, 90, 135, 180 kg N ha -1, and 135 kg N plus 40 kg P ha-1). Soil samples have been taken periodically from these plots. The analyses of these samples show that N content of the soil decreased rapidly during the initial years of the experiment; however, there has been little change in the N and organic matter content of the non-manured and non-fertilized plots since 1955. The N, P and organic matter content of the manured plots has gradually increased since 1942; analysis of soil from a 2006 sampling show that total N in the manured plots is near the levels of the native soil before plowing, P concentrations increased significantly. Organic matter content of manured plots is 65% greater then in nonmanured plots. Annual applications of 27 Mg ha-1 of manure have been adequate to maintain near-maximum grain yields with no additional fertilizer.