Carbon sequestration through conservation tillage may affect distribution of C, N and P fractions in soil organic matter (SOM). To evaluate the impact of no-till (NT) continuous corn on C, N and P partition in SOM, composite soil (fine, illitic, active, mesic, mollic Epiaqualfs) samples at 0-7.5, 7.5-15, 15-22.5 and 22.5-30 cm depth were randomly collected from 2 (NT2), 20 (NT20), and 40 (NT40) year NT and their adjoining conventionally tilled (CT) fields in Wood County, northwest Ohio. Soil samples were air-dried, processed and analyzed for pH, electrical conductivity (Ec), and total C and N contents. With 01M NaOH, the soil samples were extracted and fractionated for humic (HA) and fulvic (FA) acid associated C, N, and P fractions. Results showed that total, extractable, HA and FA acid associated C and N concentration at surface depth (0 to 7.5 cm) of NT soil have increased over time compared to CT soil. However, the C and N distribution in both HA and FA fractions did not change significantly. Both HA and FA acid associated P concentration had changed significantly in response to tillage. The effect was more pronounced in NT40 soil with an increase in P concentration in both HA and FA acid fractions. However, the HA acid fraction contained more P than FA acid fraction. Averaged across tillage systems, all the C, N and P fractions were significantly higher at surface than at surface depth of soil.