Epidemics of Fusarium head blight (FHB) can significantly reduce wheat grain yield and quality in the central and northern Great Plains of the U.S.A. Use of resistant cultivars is the most effective measure to control the disease. However, most hard winter wheat (HWW) cultivars currently grown in this area are highly susceptible to FHB. In addition, the disease screening procedure is laborious, time consuming, and costly, the progress in breeding for resistant HWW cultivars has been relatively slow with conventional methods. We used high-throughput marker-assisted backcross method to successfully transfer the major quantitative trait locus (QTL) from Sumai 3 and its derivatives into locally adapted HWW with minor FHB-resistance QTL to develop marketable FHB resistant HWW cultivars and useful germplasm lines. Three crosses were made between Sumai 3 derived soft red wheat lines and three locally adapted hard winter wheat cultivars (Harding, Wesley and Trego). Harding and Wesley are red wheat cultivars from South Dakota and Nebraska, respectively, and Trego is a white wheat cultivar from Kansas. Using marker-assisted backcross, about 80 Bc2F2 plants homozygous for the 3BS QTL were selected from each backcross population based on closely linked markers to the 3BS QTL. All selected Bc2F3 lines were evaluated in the greenhouse for Type II resistance in the USDA Genotyping Center in the fall of 2006. The result indicated that 135 lines were highly resistant and 87 lines were moderately resistant. These materials have also been planted in mist-irrigated fields for further selection of FHB resistance, winter hardiness, hard-textured grains, and other traits at the three locations. Some lines with good FHB resistance and other desirable traits will be released as new germplasm or cultivars in hard winter wheat grown region after further yield trials.