Soybean Mosaic Virus (SMV) is one of the most prevalent viral pathogen affecting soybean [Glycine max (L.) Merr.] yield and quality. Genetic diversity of soybean with resistance to SMV is necessary for selection of parents in a breeding program to maximize genetic improvement. The objective of this research is to analyze genetic diversity in soybean genotypes characterized in previous studies as resistant to SMV on the basis of their differential reactions to SMV-G1 and SMV-G7 strains.One hundred and fourteen genotypes resistant to SMV collected from USA, China, Korea, and Japan were screened by using 139 Simple Sequence Repeat (SSR) primers covering the 20 molecular linkage groups (MLG) of soybean. Results showed that 113 primers were polymorphic among 114 genotypes. Genetic similarity between pairs of genotypes, gene frequency, genetic diversity index, and cluster analysis results will be presented.