In this investigation, we observed the physical and chemical properties of a soil on pine mushroom picking areas where were located in the most upper and lower parts representing a comparative climatic characteristics in Korea. The differences in slope gradients of the investigation areas which was divided into 100 quadrates were 8.6°(left to right) and 22.7°(top to bottom) and 6.8°(left to right) and 17.8°(top to bottom) for Ponghwa and Gansung, respectively. The amount of clay and thickness of organic layer in the top soil were significantly decreased with increasing slope gradient, resulting in decrease of the soil moisture content around a fairly ring-colony of Tricholoma matsutake which was generally observed under the relatively thicker organic matter layer beyond 3 cm depth. pHs of the soil were weak acid and average EC was 0.44 dS m^-1 in both areas. The cations were in the order of Fe K > Na > Mg > Ca and Fe > K > Na > Ca > Mg for the upper(Gansung) and the lower (Ponghwa) part. And the amount of Fe was approximately 80 mg kg^-1 or greater in the pine mushroom picking soil. From this, we could assume that the growth of the pine mushroom was closely related not only with iron but also soil moisture content.