Glyphosate resistant (GR) soybean contains a resistant EPSPS enzyme enabling the shikimate pathway to proceed, following glyphosate exposure, without inhibition. Yet residues of glyphosate and its metabolite, aminomethylphosphonic acid (AMPA), have been detected in GR soybean seed signaling glyphosate degradation within the plant. Leaf chlorosis has been frequently observed in GR soybean following glyphosate applications. During 2004 and 2005, we applied glyphosate and ammonium sulfate (AMS) or AMS alone to irrigated soybean. Following glyphosate applications, frequent SPAD meter measurements were taken on the uppermost developed trifoliates. These same trifoliates were also analyzed to derive chlorophyll absorbance values. These absorbance values were converted to chlorophyll and nitrogen concentrations. Leaf chlorosis was confirmed in plants which received applications of glyphosate and AMS; up to a 7% reduction occurred relative to the control. Longevity of the detected chlorosis was greatest when glyphosate and AMS were applied at the time of flowering (R1/R2). Glyphosate, or a derivative, appears to have a direct role in inducing chlorosis within the soybean and implications of this are explored.