Tthis study was investigated the effect of N (0, 67, 135 and 202 kg/ha), cultivar (DK3875, DKF2990, DKF3510 and DKF3901) and their interaction on seed yield, oil content, and composition of sunflowers grown at five locations in Mississippi (Newton, Starkville, Stoneville, and lowland and upland locations in Verona). The tested cultivars had dissimilar fatty acid composition. The concentration of oleic acid in the original seed oil of the four cultivars DKF3875, DKF2990, DKF 3510 and DKF3901 was 290, 260, 850 and 410 g/kg, respectively. Cultivar and N rate were significant with respect to yields at Stoneville, Newton and Verona upland, N rate was significant at Stoneville, and cultivar x N interaction were significant at Starkville and Verona lowland. In Stoneville, Newton, and Verona upland, DKF3510 provided the highest yields. DKF2990 provided lower yields in Stoneville and in Verona upland. The seed oil concentration was higher in DKF3875 and DKF2990 (420-460 g/kg) and lower in DKF3510 and DKF3901 (400-430 g/kg) at Stoneville and Verona upland. However, at Newton, the oil concentration was highest in DKF2990, lower in DKF3510 and DKF3901 and lowest in DKF3875. With one exception, oleic acid increased in all locations and cultivars relative to the concentration of this acid in the original seed. As expected, there was a corresponding decrease in the concentration of linoleic acid. Also, the concentration of stearic acid decreased relative to the original seeds. These results suggest that sunflowers under the hot humid climate of Mississippi may accumulate a higher concentration of oleic acid and a lower concentration of linoleic and stearic acids. In most cases, N rate, cultivar, or N x cultivar interaction altered the concentration of other fatty acids (palmitic, arachidic, gondoic, behenic, and lignoceric) in sunflower oil. Sunflowers can be a viable crop in most parts of Mississippi.