The effective interaction between soybean genotypes and Bradyrhizobium japonicum inoculants are limited by the ability of these strains to compete with the relatively mediocre indigenous soybean bradyrhizobia strains. In this study, Rhizobiophage PA14 which was found to have the widest host range among phages isolated from the rhizosphere soil of soybean grown in the Eastern Shore of Maryland, was used to examine the potential of using lytic rhizobiophages (Phage PA14) along with phage-sensitive (USDA 110) and phage-resistant (USDA TA11NOD+) bradyrhizobia strains to limit soybean nodulation by indigenous undesirable bradyrhizobia and thus, enhance the nodulation by the effective inoculant strain, TA11NOD+. The inoculant which contained phage PA14 and strain USDA 110 suppressed nitrogenase activity by 49%, while no significant difference was shown when the inoculant contained USDA TA11NOD+ with or without phage PA14. Further, nodule occupancy by the superior inoculant strain, USDA TA11NOD+, was increased by 59% when soybean seeds were coated with USDA TA11NOD+ and Phage PA14. When soybean seeds were coated with PA14 and USDA 110, nodule occupancy by USDA 110 was decreased by 78%. This study indicates that phage PA14 in the presence of a compatible phage-resistant bradyrhizobia inoculant has the ability to significantly decrease the occupancy of mediocre bradyrhizobia strains. Therefore, phage PA14 can be used as a potential biocontrol agent to partially eliminate mediocre yet competitive B. japonicum strains and, thus, enhance nodulation, biological nitrogen fixation and growth of soybean by the desirable strain.