The enzymatic activity of ECM may play an important role in nutrient mobilization and transfer from the soil solution and soil organic matter (SOM) into the plant host. In the classical view, the plant provides C derived from photosynthesis and in return, the fungi provide the plant with water and soil-derived nutrients such as N and P. Although ECM fungi are believed to acquire their C from the plant partner they have traits in common with saprotrophic fungi and may enable them to break down SOM. ECM fungi can produce extracellular enzymes such as glucosidases, cellulases, lignin peroxidases, polyphenol oxidases, chitinases, proteases and phosphatases. In order to assess the functional diversity of ECM we studied the ability of ECM to degrade SOM. Enzymatic profiles were generated for ECM tips from pines from three different Pinus ponderosa plantations that differed in terms of climate, soil chemistry, site index and nutrient availability, particularly N and P. In each of the sites we had two treatments, a control and a N-exponential fertilization applied for ten years after plantation. ECM root tips were harvested and classified by their morphological characteristics into five predominant morphotypes. Using ECM root tips, we measured activity of these three enzymes: glucoronidase, leucine aminopeptidase and acid phosphatase. Enzymatic activity was measured for individual ECM root tips in a microplate based assay utilizing fluorescent substrates. Enzymatic profiles differed by ECM morphotypes, by treatment, non-fertilized vs. fertilized and by site. These findings indicate that ECM fungi can produce different enzymes in response to environmental conditions.