As biodiesel production increases, significant boost in canola acreages is being projected worldwide. In the southeastern United States, winter canola is planted in the fall and harvested in late spring. Mild winter conditions allow canola to grow rapidly, while occasional spring frosts can damage cultivars at vulnerable growth stages. In this paper, responses to freezing temperatures by sixty winter canola genotypes during flowering and early seeding are reported. Frost damage to genotypes at different stages of reproduction exposed to three-day severe frost (-5.5, -5.5 and -0.5 °C) in April was evaluated. Entries were from the National Winter Canola Variety Trials, planted at the Alabama A&M University Winfred Thomas Agricultural Research Station (latitude 34° 35'N). Experimental plots were in six rows, 6m long with 18cm row spacing, planted using conventional practices which include: pre-plant herbicide treatment of trifuralin at 0.2 l ha^-1, 60 kg ha^-1 of 13-13-13 (N-P-K) six weeks after planting; and split application of 180 kg ha^-1 of 34-0-0. Phenotypic observations indicated varied responses to stem breakage, scorching of leaf, bleaching of green tissues, flower abortion and empty pods among the genotypes. Preliminary damage assessment showed significant number of empty pods in the early-maturing entries, cessation of growth of fertilized ovules in mid-maturing entries, and drop of unfertilized flowers in late-maturing entries. Post harvest agronomic data including pods plant-1, seeds pods-1, seed yield, test weight, and oil content will be discussed in relation to freezing temperatures and variations in maturity among winter canola cultivars.