The PI3848 triticale from Russia is among the highest resistant line to the RWA (types 1 and 2). This triticale line was used as the male parent in crosses with Lamar wheat. The F1 plants were backcrosses to Lamar wheat. The RWA resistant BC1F1 were backcrossed to the same recurrent wheat parent. After the second backcross, the interspecific hybrids were selfed and the progenies tested for RWA reaction for at least eight generations. Five lines from these selections were identified for their resistance to the RWA and their seeds were increased for agronomic and other characterizations. Molecular and cytological analyses of these lines were performed using GISH and chromosome- specific microsatellites markers. Two lines were cytologically stable and carried a pair of rye (Triticum strictum) chromosome as addition lines. Three other lines were instable and showed a high level of myxoploidy. No wheat –rye chromosome interchange was detected suggesting little homology between T. strictum and T. aestivum chromosomes. Specific microsatellite primers were used to identify the rye chromosomes present in each line. Meiotic behavior of the rye chromosomes in Lamar wheat background is being investigated.