The paper deals with the process of selecting the strains of multi-functional bacteria. In consideration of the impact on the environment by the release of genetic engineering strains, the screening of natural multi-functional bacterium strains is of particular importance. By inoculating the medium with Ca₃ (PO₄) ₂ and lecithin as the only P source respectively with the strains of bacterial fertilizer in the laboratory, 9 strains of phosphorus-dissolving bacteria were selected. Then a test of the invert intensity was made. In comparison with the controls, the contents of available phosphorus increased from 33.122μg.ml^-1 to 343.86μg.ml^-1 in the case of the medium with Ca₃ (PO₄) ₂ as the only P source.While the contents of available phosphorus increased from 0.720 to 42.973μg.ml^-1 in the case of the medium with organic P lecithin as the only P source. The 9 strains screened out went through an antagonistic test with 3 strains of pathogenic microbes (Erwinia sp.,Pseudomonas sp.,Xanthomonas sp.), 2 multi-functional strains of Micrococcus sp. and Pseudomonas fluorescens Migula KO were selected that could dissolve phosphorus, restrain bacterium and increase seedling growth. Key Words: Bacterial manure, Inorganic phosphorus, Organic phosphorus, Phosphorus-dissolving bacteria, Available phosphate, P-release, Antagonistic effect, Multi-functional strain