Qingzhen Jiang ¹, Justin Clawson ¹, David Hole ¹, Pat Hayes ², Ann Corey ² and Dominique Roche ¹.

1- Department of plants, soils and biometeorology, Utah State University, Logan, UT 84321, USA 2- Dept. of Crop and Soil Science, Oregon State University, Corvallis, Oregon, OR 97331, USA

Protein polymorphism was investigated in water-soluble seed extracts between Hordeum vulgare subsp vulgare (Harrington cv.) and Hordeum vulgare subsp spontaneum. Analysis of polymorphic spots by tandem mass spectrometry (LC-MS/MS) led to the identification of gene products of CMa, a component of a tetrameric inhibitor to an insect á-amylase. In H. v. vulgare barley, the CMa gene is located on chromosome 1(7H) bin 4 (SSR HvCMA). Preliminary mapping on a partial set of recombinant chromosomal substitution lines (27 RCSLs) confirmed a position in a H. v. spontaneum genomic region homologous to HvCMA. We will present the experimental design to test some RCSLs polymorphic for this protein in presence of a heavy infestation of cereal leaf beetles (Oulema melanopus). This example seems to confirm that the proteomic approach may yield some wild/donor alleles from H. v. subsp. spontaneum for barley improvement.