Monday, 7 November 2005
6

Use of Marker-Assisted Selection(Mas) of Resistance to Brown Planthopper in Rice(Oryza Sativa L.).

Jeom Ho Lee V1, Oh Yeong Jeong1, Yong Hee Jeon1, Sae Jun Yang2, and Young Tae Lee2. (1) National Institute of Crop Science, RDA, Suwon, South Korea, (2) Institute of Crop Science, RDA, Suwon, South Korea

The brown planthopper(BPH), Nilaparvata lugenes Stal, is the most significant insect pest of tropical as well as temperate region, throughout rice growing countries. This experiment aimed to evaluate a molecular marker for screening of brown planthopper resistance in rice. Seedlings of four recombinant inbred populations developed from crosses between an authentic Bph1 resistant gene introgression lines and susceptible lines were subjected to greenhouse feeding screening with biotype I BPH for phenotype evaluation. From the same plants, DNAs were isolated and amplified using DNA markers linked to resistance(R) genes, pBPH9 primer developed by Dr. Cha et al.(2004). The consistency between the presence of marker and resistance in phenotype evaluation was highly correlative in all cross combinations (mean 94.9%, range 90.4`98.7). This implie the pBPH9 primer used for the experiment may replace the conventional laborious feeding screening in greenhouse for the selection of BPH resistant lines in beginning of breeding procedure. One interesting finding was the indica inbred showed much more higher consistency than that of japonica inbred. This may be due to the gene depression in late generations of japonica inbreds or loss of the gene action in japonica inbreds while active in indica inbred. These DNA markers linked to gene resistance to the BPH biotype of Korea could be application for MAS of BPH resistance lines in rice breeding programs in Southeast Asia.

Back to Marker Applications in Breeding
Back to C01 Crop Breeding, Genetics & Cytology

Back to The ASA-CSSA-SSSA International Annual Meetings (November 6-10, 2005)