ABSTRACT The influence of agricultural practices on greenhouse gas generation and emission is of interest due to its potential to contribute in global climate change. Agricultural ecosystems can play a significant role in production and consumption of greenhouse gases CO₂, CH₄ and N₂O. The objective of this study is to investigate how tillage practices, weather parameters and soil properties interact to influence greenhouse gases emission in agricultural ecosystem. The experiment was conducted on the long term tillage experimental plots consisting of no till; chisel plow and moldboard plow plots located at South Charleston, Ohio. Gas sampling was initiated in April 2005.Gas samples were collected using closed soil chamber method for 1 hour at 30 minutes intervals and CO₂, N₂O and CH₄ concentrations were determined by gas chromatography. Generally greater fluxes of N₂O and CO₂ were observed after rainfall events and during the days immediately after fertilizer application. Methane fluxes were generally higher in the no till plots than moldboard and chisel plow. Overall, the GHG emissions varied with soil conditions, and tillage methods.