A two-year (2003-04) study was conducted on a Marietta silt loam and Falaya silt loam soils in North Mississippi, evaluating cotton lint yield and HVI fiber quality response to spindle picker narrow and wide row spacings. The row patterns used in the study were 15, 30, 38 and 60-inch row solid cotton. The skip row systems were 15, 30 and 38-inch rows with a 2 x 1 skip row pattern, and a 15-inch row 2 x 2 skip row pattern. The plots were harvested with a John Deere PRO 12 VRS spindle picker row unit mounted on a single row picker chassis (John Deere model 122 one row cotton harvester) mounted on a gear drive John Deere 4020 tractor. The seed cotton samples were ginned with a small-scale gin (equivalent to a commercial gin). The first fruiting branch average node location was 6 and was not affected by row patterns, years and locations. The 15-inch row two-year (2003-2004) average lint yields of 1120 and 1150 lb/A on the Marietta and Falaya soils were 108 and 60 lb/A more than the 30-inch row, respectively. However, the 15-inch row yield was not significantly different from the 30-inch row or 15-inch 2 x 1 skip row pattern on both soil types. The 15-inch row solid yield was greater than 38 and 60-inch row, 38 and 30-inch 2 x 1 skip row patterns and 15-inch 2 x 2 skip row pattern on both soils. The yield average for 2003 was greater than 2004 on the Marietta soil and greater for 2004 than 2003 on the Falaya soil. However, there was no year by row pattern interaction for yield on both soils. There also were no meaningful fiber length, uniformity, strength, micronaire, Rb and +b fiber quality differences between treatments and years at both locations.