Long-term use of chicken litter manure in farmlands has caused a sharp increase in the concentration of soil and water phosphorus (P). Movement of excess soil P through runoff and leaching results in eutrophication of waterways leading to the deterioration in water quality. Lolium multiflorum (Gulf and Marshall ryegrass) has been shown to accumulate high concentrations of P from KH2PO4-enriched media. In order to test the ability to remove P from chicken litter-contaminated media, Gulf and Marshall ryegrasses were grown in aqueous medium and soils containing 100g chicken litter/L or Kg, respectively, in the presence of different chelators (EDTA, HEDTA, NTA). Plants were grown in a greenhouse and harvested after 2 weeks (aqueous medium) and 4 weeks (soils). Plants tolerated high concentrations of chicken litter and chelators, and increased in biomass significantly. P accumulations in roots and shoots are being determined.