Canola (Brassica napus L.) has relatively high nutrient requirements; therefore, information on nitrogen and sulfur relationship in its production is very important. In southeastern states, canola production has potential, however, data on the requirements of nitrogen and sulfur for its production is not available. Therefore, research to determine the effect of various nitrogen rates (50, 100, 150, and 200 lb/a) and sulfur rates (0, 10, 20, and 30 lb/a) were evaluated on oil content and yield in canola. The highest oil content (43.8%) was observed at 50 lb/a nitrogen rate. Sulfur rates did not influence the oil contents although it decreased with an increase in nitrogen rates. The fatty acids profile and saturated fat were not significantly affected by nitrogen and sulfur rates. Significant nitrogen rates by sulfur rate interactions were observed for seed yield, number of pods per plant both in 2003 and 2004 growing seasons. Seeds per pod, pods per plant and plant height were significantly correlated with seed yield. Among the nitrogen rates, the highest seed yield of 2969 kg-1 was observed at 200 kg-1 nitrogen rate.