Fusarium head blight (Fusarium graminearum, Schwabe; FHB) is a devastating disease affecting all classes of wheat (Triticum aestivum L.) crops. A field study with spring wheat was conducted to assess how management practices (planting dates and N application treatments) affect incidence and severity of FHB, and if harvesting time affects the accumulation of deoxynivalenol (DON) in the grain. From physiological maturity, weekly samples were taken for the determination of DON in the grain. Our data showed that late planting increased incidence of FHB in the field, and both late planting and delaying harvest after physiological maturity increased significantly DON concentration in the grain. This study indicates that timely planting and harvesting may serve as practical measures to reduce DON concentration in wheat grain.