Perennial ryegrass (Lolium perenne) is one of the most important forage crops in Europe and Australia and also a popular turfgrass in North America. Improvement of resistance to multiple diseases is desirable for perennial crops like turfgrass that have long rotation cycles. Quantitative trait loci (QTL) analysis based on a three-generation interspecific ryegrass population was conducted to define partial resistances to four different fungal diseases: leaf spot (Bipolaris sorokiniana), gray leaf spot (Magneporthe grisea), crown rust (Puccinia coronata), and stem rust (Puccinia graminis). A total of 16 QTLs conferring disease resistances to these four pathogens were mapped on six of seven genetic linkage groups (LG) of ryegrass. These disease resistance QTLs were compared with disease resistance loci previously identified in cereal crops, using the rice physical map as a reference map. Most of the pathogen-specific QTLs identified in ryegrass also occurred at corresponding positions in cereal crops. One genomic region associated with multiple disease resistances was found on ryegrass LG 4, which has a syntenic relationship with a genomic region of rice chromosome 3 where broad-spectrum resistance loci were found. Results of this comparative QTL analysis using the integrated map of both the ryegrass genetic map and the rice physical map indicated the conservation of pathogen-specific partial resistance between ryegrass and cereal crops as well as of broad-spectrum resistance to multiple diseases. Therefore, genetic information on disease resistance genes identified in rice or other cereal crops can be readily transferred into understudied turfgrass so that breeding for multiple disease resistant cultivars can be greatly facilitated via marker-assisted selection and gene pyramiding approaches.