There is increased concern over N2O emissions from arable crops such as potato (Solanum tuberosum L.) which receive high fertilizer N inputs. This study examined the effect of the rate and timing of fertilizer N application on potato on N2O emissions in two years. Treatments included a control with no fertilizer N applied, addition of 200 kg N ha-1 banded at planting similar to current grower practice, and split N application with 120 kg N ha-1 banded at planting plus 80 kg N ha-1 banded at final hilling. N2O flux was measured separately in the potato hills and furrows approximately weekly using non-flow-through, non-steady-state chambers. Denitrification rate was measured approximately monthly using the acetylene inhibition method. The pattern of N2O flux over time was consistent with N2O emissions occurring primarily as a result of denitrification. Nitrogen fertilization increased cumulative growing season N2O emissions in both years.