Tuesday, November 14, 2006

Analysis of Quantitative Trait Loci for Seed Coat Cracking from Two Soybean Populations.

Sung-Taeg Kang1, Min-Jung Seo1, Jung-Kyung Moon1, Hong-Tae Yun1, Seuk-Ki Lee1, Yeong-Ho Lee1, Si-Ju Kim1, and Hyeun-Kyeung Kim2. (1) National Institute of Crop Science, R.D.A., 209 Seodun-dong, Suwon, South Korea, (2) Medical Research Institute, Busan National Univ, 10 Ami-dong 1ga, Busan, South Korea

Seed Coat Cracking (SCC) in soybean showing cracks in the surface of seed coat, causes serious effect on seed quality in soybean. However, breeding for resistance to SCC is difficult due to the complicated genetic behavior and environmental interaction. The objective of this research was to improve breeding efficiency for resistance to SCC based on DNA marker in soybean. In this study, SSR markers were used to identify the genomic regions significantly associated with the quantitative trait loci (QTL) that controls SCC in two segregating F2:11 RIL populations, 'Keounolkong' / 'Shinpaldalkong' (K/S) and 'Keounolkong' / 'Iksan10' (K/I). The two populations were phenotyped at two locations in Suwon and Yeoncheon, Korea. In the K/S population, four QTLs for SCC were identified at Suwon, six at Yencheon, with total phenotypic variations of 6.68-12.18% and 3.65-11.69%, respectively. Two QTLs were commonly identified on the linkage group D1b and I at two locations, accounting for over 10% of the phenotypic variation. In the K/I population, three QTLs for SCC were identified at Suwon, four at Yeoncheon with total phenotypic variations of 5.26-8.45% and 3.26-11.23%, respectively. QTLs on D1b and D2 were commonly associated with SCC at two locations. Especially QTL tightly linked with Satt216 on LG D1b was commonly associated with SCC across populations and locations. Results showed that major QTL for SCC has been identified on LG D1b, D2, and I. And these major QTLs for SCC may be used for minimizing soybean SCC through effective marker-assisted selection (MAS).

Handout (.ppt format, 323.0 kb)