Monday, November 13, 2006

Haplotyping Wheat Lines for FHB Resistance.

Charlie Zila, Purdue Univ, Lilly Hall, 915 W State St, West Lafayette, IN 47906, Xiaorong Shen, Purdue Univ, 2286 Cousteau Dr., West Lafayette, IN 47906, and Herbert Ohm, Purdue Univ, Agronomy Dept, 1150 Lilly Hall, Room 1-300, West Lafayette, IN 47907-1150.

Fusarium head blight (FHB) is a devastating disease of wheat (Triticum aestivum L.) that causes reduced grain yield and causes infected grain to be unfit for consumption due to mycotoxins produced by the fungus.  The objective of this study was to determine the potential novelty of FHB resistance of two new wheat lines, X117 and 4153 by comparing their haplotypes to the haplotypes at marker loci associated with FHB resistance of other resistant wheat lines.  X117 and 4153, and  the seven  FHB resistant wheat lines with previously mapped resistance - Ning 7840, Frontana, Wangshuibai, Arina, Renan, F201R, and Chokwang, along with the FHB susceptible wheat lines 9762 and 9774 (crossed to X117), and P92226 and P981312 (crossed to 4153), were haplotyped.  DNA from each of the above 13 wheat lines was amplified by polymerase chain reaction (PCR) using primers for the respective DNA markers associated with the known FHB resistance QTLs.  Amplified DNA was separated on 3% agarose gels and viewed under ultraviolet light.  X117 was polymorphic with all FHB resistance markers, except the resistance of F201R.  Line 4153 was polymorphic with all FHB resistance markers, except the resistance of Frontana.  This indicates that X117 and 4153 possibly share resistance genes, respectively, with F201R or Frontana.  However, in previous repeated tests, the level of FHB resistance of X117 is greater than that of F201R and the resistance level of 4153 is greater than that of Frontana. Thus, X117 and 4153 likely have novel FHB resistance genes. X117 and 4153 each have been crossed to two FHB susceptible lines to produce recombinant inbred populations, currently in F3 generation, with the goal of identifying and mapping their FHB resistance.