Anthocyanins Profile and Antioxidant Activity of Colored Barley Germplasms.
Mi-Jung Kim1, Jin-Ae Kim2, Min-Young Kim2, Ill-Min Chung2, Jong-Nae Hyun1, and Jung-Gon Kim1. (1) Honam Agricultural Research Institute, 381 Songhak-dong, Iksan, Jeonbuk, South Korea, (2) Konkuk Univ, 1 Hwayang-dong, Gwangjin-Gu, Seoul, South Korea
Barley is recommended for healthy diets due to its high fiber content and antioxidant substances. Colored barley is also known to contain anthocyanins and anthocyaninshave antioxidant.In the present study, twenty-two lines of colored barley germplasms including ten lines of black barley, ten lines of purple barley and two lines of blue barley which were screened for their anthocyanins profile and the antioxidant activity.Separation of anthocyanins by high-performance liquid chromatography (HPLC) showed that each colored barley had a distinct anthocyanins profile. Antioxidant activity of colored barley was evaluated on the basis of scavenging capacity of 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radicals. Five major anthocyanins were separated from purple and blue barley as compared to four anthocyanins in black barley. The most common anthocyanin in purple barley was cyanidin-3-glucoside and in black and blue was barley delphinidin-3-glucoside. The purple group had higher total average content of anthocyanins and the DPPH radical scavenging activities than those of colored barley. Colored barley germplasms should be very useful in breeding for healthy foods.