The degradation of water quality due to anthropogenic activity is destroying both recreational and natural values of waterways worldwide. Some agricultural practices may be largely responsible for this degradation. The problem is becoming so severe that waters in the St. Cloud area are unsafe for recreational purposes. Animal feedlots are sites intended for confined feeding, raising, or holding of livestock. These areas of confinement result in an accumulation of manure and often limited vegetation (MPCA, 2003). The lack of vegetative cover and manure accumulation combine to create a number of surface runoff issues, most notably coliform contamination, which can be harmful to human life. A monitoring study conducted between 2005 and 2006 by the author of this study concluded that rainfall events contributed dangerous levels of E. coli bacteria to the Sauk River. While the previous study focused only on water quality, this study will shift focus to coliform contamination and its relationship with animal feedlots. Animal wastes from feedlots may contain harmful coliform bacteria, such as fecal coliform and E.coli, the latter of which is a direct threat to human health. On September 16, 2006, 17 sites along the entire stretch of the Sauk River were examined for coliform contamination. Findings from this study have been analyzed using GIS software to investigate any correlation between location of animal feedlots and levels of coliform bacteria.