Tuesday, November 6, 2007 - 10:00 AM
167-5

In Vitro Response of Cyanobacteria Common in Creeping Bentgrass Putting Greens to Micronutrients.

Steven Borst, J. Scott McElroy, Steven M. Wilhelm, and John C. Sorochan. 2431 Joe Johnson Drive, University of Tennessee, University of Tennessee/ Turfgrass Weed Science, 252 Ellington Plant Science Building, Knoxville, TN 37996-4561

Cyanobacteria encroachment on creeping bentgrass (Agrostis palustris) putting greens is a common problem. Chemical control methods have varied in effectiveness. Mercury has been restricted from pesticide formulations but other plant nutrients such as copper and zinc have been added as a replacement in these formulations. Heavy metals such as copper (CuSO4) have been utilized in aquatic situations to control Cyanobacteria blooms. An In vitro response screen utilizing six different concentrations of CuSO4 and ZnSO4 (0, 0.63, 3.15, 6.3, 9.45, 12.6 µM) was administered to Phormidium spp.. Each concentration was added to 30 ml of standard BG-11 media and inoculated with Phormidium spp. filaments. Triplicates of each concentration were then harvested at (0, 3, 5, 7 and 10) days after inoculation and chlorophyll amounts were measured.