A number of studies have used NMR to characterize water dissolved organic phosphorus (DOP) in animal waste. However, most of the work up to date has focused on characterization of organic P that is extracted using concentrated NaOH or EDTA/NaOH. Little information is available on DOP species in animal wastes. Our previous research on poultry wastes has shown that the concentration of DOP can increase between 70% and 3000% -when extracted at pH 6 compared to extraction at the unmodified pH of the waste. In this research we used NMR to characterize DOP extracted from broiler litter and layer manure by sequential extraction, original pH using deionized water, pH 6 buffered using 0.1 M 2-(N-morpholino) ethanesulphonic acid (MES), pH 9.2 using 0.5 M NaHCO3, pH 11.22 using 0.1 M NaOH, and pH 0.75 using 1 M HCl. Extracts were freeze-dried and subsequently redissolved in deuterium oxide and 0.5 M NaOH. The 31P-NMR spectra were acquired on a Varian Inova 500 MHz spectrometer (11.75T; 202.5 MHz for 31P) without proton decoupling. An acquisition time of 0.8s, delay time of 5s, 450 pulse and 4000 scans were used in this experiment. At original poultry litter pH main inorganic species in water soluble P (WSP) was orthophosphate and main DOP species was orthophosphate diester and phospholipid. At pH 6.0, a poultry litter extracted with MES buffer showed the orthophosphate monoester was the main DOP species in WSP. The main species in WSP from a layer waste was orthophosphate. Orgnaic P speciation as revealed by NMR in extracts of MES, DI water, NaHCO3, NaOH, and HCl will be further discussed.