Atrazine has been used leading herbicide widely applied in the US and Mid Western states. Recently, public health and ecological concerns have been raised about contamination of surface and ground water by the persistent ATR and its chlorinated metabolites, due to their toxicity and potential carcinogenic or endocrinology effects. Phytoremediation been proven to be one of the most cost-effective mitigation practices to remove atrazine from surface runoff derived from agronomic operations. Current ongoing research have identified several plant species showing promising capacity degrade most of the soil atrazine to less toxic or less mobile metabolites in the rhizosphere . However, The mineralization of atrzaine and its chlorinated metabolites or completed cleavage of trazine ring in the rhizosophere, was limited less than 2-10% under both laboratory and field conditions. Despite of the persistency of atrazine and its degradates in the environment, a few bacteria strains including Pseudomonas sp. ADP were isolated in the past decade from the atrazine spill site heavily contaminated. These bacteria contained a series of genes encoded on a self-transmissible plasmid pADP-1 responsible for various processes of atrazine degradation resulting in completed ring cleavage that rapidly mineralize the atrazine into carbon dioxide. We proposed to investigate the synergic effect of introducing these biological agents on enhance the phytoremediation of atrazine. The developed knowledge will be transferred for future field application to reduce the atrazine and its metabolites migrate to water sources.