The soil resource is an integral part of sustainability.  Management dependent properties describe dynamic soil quality, however, comparisons of disturbed to reference sites are not extensive in the SE due to scarcity of undisturbed land.  Objectives of this study are to evaluate land use and management effects on dynamic soil properties of southeastern soils and investigate carbon (C) stocks and soil quality of mature longleaf-wiregrass habitat relative to more intensively cultivated Coastal Plain ecosystems. Sites in Thomas County, GA, representing three soil map units (sandy surfaces with loamy to clayey kandic subsurface horizons) were selected in each of three management systems for comparison of near surface (0-5, 5-15 and 15-30 cm) soil chemical, physical, and biological properties.  Land management/use included mature longleaf pine (Pinus palustrus Miller) – wiregrass (Aristida stricta Michx.) habitat (LL), slash pine (Pinus elliottii Engelm.) plantation (PP), and conventional row cropping systems (RC).  Microbial biomass C (0-5 cm) was 57 and 101 % higher in LL compared with PP and RC, respectively, while total organic C was 68 and 148 % higher in LL relative to PP and RC, respectively.   Anthropogenic inputs were evident in RC (0-30 cm) based on high TON (21 and 28 % > LL and PP), Ca (92 and 186 % > PP and LL), Mg (57 and 50 % > LL and PP), K (180 and 600 % > PP and LL), P (204 and 1600 % > PP and LL), and base saturation (107 and 148 % > PP and LL).   Bulk density was highest in RC across all depths with the greatest differences at 0-5 cm (RC 19 % > PP) and 5-15 cm (RC 34 % > LL).  More intensive cultivation increased nutrient levels and soil compaction, and decreased C stocks of investigated soils relative to uncultivated sites.