The effect of water deficient stress on total non-structural carbohydrates in too sainfoin species (Onobrychis radiata & Onobrychis viciifolia) was surveyed .Experimental design was a RCD with 4 replication. The plants under study were in the greenhouse environment with the maximum temperature of 38ēC and the minimum temperature of 14ēC. Forty days after the cultivation, both types were put under the care of water deficient stress based on the subtraction from the rate of moisture in the farm 75% field capacity (FC) and 50%FC, and samplings were carried out in germination and reproduction stages, with irrigation level of 100%FC being taken as control. The biochemical surveys showed that root and shoot nitrate contents in both species increase under stress conditions as compared to that of control. O.radiata contained higher osmosis metabolites, such as nitrate than O.viciifolia, under stress.

Key words: Sainfoin, Water stress, Field capacity(FC), Osmosis  metabolites