Monday, November 5, 2007
59-10

Equipment for Inoculation of Bacterial Pathogens.

Joseph T. Metzler, Mycogen Seeds, PO Box 139, Sidney, IL 61877

There has been a desire for the screening of inbreds and hybrids against the bacterial pathogens causing Stewarts Wilt and Leaf Blight (SWLB) Erwinia stewartii and Gosses Wilt (GW) Clavibacter michiganensis subsp. nebraskensis. The traditional inoculation method using “clappers” is still being used. The clapper uses a bed of nails embedded in an inoculum soaked sponge on the bottom of both wings to wound and deliver the inoculum. It can produce reliable ratable results but is labor intensive and suffers from built in inoculator and worker variation. A field ready prototype was developed that simultaneously wounds and inoculates the plant. The fragile bacterial cells are not damaged in the process and bacterial inoculum is gently forced into the cut edges of the wounds. This provides reliable ratable results and reduced labor requirements and human error.