To identify desired soybean oil and meal traits, the soybean research community has utilized for many years both the vast soybean germplasm collection and traditional forward genetic approaches in combination with phenotypic screening. Much progress has been made, and recent use of candidate gene approaches has revealed the molecular genetics underlying a subset of those oil and meal traits. Other traits have proven less tractable, with complex genetics and difficult introgression into elite lines, possibly due to the reliance on phenotyping as the primary screen. In the era of complete genome sequence of several plant genomes, the utility of reverse genetics for crop improvement must be determined. The goal of a reverse genetics project is also to discover lines with a particular trait, but the approach relies on identifying a target genotype rather than phenotype. Screening is done on germplasm collections or mutant populations for polymorphisms in the target genes of interest followed by characterization of the association of the predicted phenotype with the target gene polymorphism. The objective of this project is to develop reverse genetic resources for soybean seed composition improvement using EMS and fast neutron mutant populations.