NECi (The Nitrate Elimination Co., Inc.) has developed a variety of enzyme-based nitrate analysis methods for agricultural samples using recombinant Nitrate Reductase (Superior Stock AtNaR2). These methods are sustainable and Green due to the use of biodegradable reagents. NECi has developed Nitrate Test Kits (NTK's) for extracts from soil, petioles, leaves, dry forage, and standing crops such as hay, wheat, and corn silage. These kits, which are fast and easy to use, are designed to provide reliable nitrate values on-site, in the field, to enable more accurate decision-making. The Ag-NTK's can be used in NMP compliance, PSNT testing, to make harvest and nutrient application decisions, and to protect livestock from nitrate toxicity. They function best to provide supplemental data in conjunction with quantitative results from commercial or Extension testing labs. For more definitive nitrate content analysis, NECi has collaborated with EST Analytical for development of an automated enzymatic reduction method using AtNaR2 in the new discrete analyzer technology, specifically the KoneLab Aquakem instrument. The automated method yields nitrate content results equivalent to the widely used cadmium reduction method. The new discrete analyzer (DA) technology provides high throughput with as many as 600 samples handled per hour. DA's require only very small volumes – microliters - of reagents and sample, yielding cost savings in reagent and sample transport. In addition, small volume minimizes waste. The nitrate analysis methods have also been adapted for Total N analysis using alkaline persulfate digestion to convert organic N to nitrate. When combined with nitrate analysis of the same samples, results equivalent to Kjeldahl analysis are obtained. Thus, enzyme-based nitrate analysis is a Green method with versatile applications in agriculture. (Partially funded by grants from US Dept of Agriculture and State of Michigan Dept of Agriculture)