The production of cultivated peanut, an important agronomic crop throughout the United States and the world, is consistently threatened by various diseases and pests, such as Sclerotinia blight, caused by the fungus Sclerotinia minor Jagger. Furthermore, the peanut industry in the Southwestern U.S. currently demands varieties with high oleic acid content. In order to accelerate the development of disease resistant, high oleic peanut varieties, we are developing a multi-component system which allows screening of F1 hybrids for desired traits. First, the oleic acid content of single peanut seeds is determined using near infrared reflectance (NIR) analysis, which is non-destructive and allows for subsequent germination and plant generation. Second, individual peanut seed are genotyped using simple sequence repeat (SSR) molecular marker associated with resistance to Sclerotinia blight. Third, greenhouse testing is performed on detached F1 shoots for resistance to S. minor enabling the identification of promising hybrids without eliminating the production of F2 seed. The objective of this study was to examine F1 populations for desired traits using the approach outlined above. Preliminary results indicate this multi-component approach is effective for identifying the presence of desired traits in the F1 populations tested. Future use of this approach to screen segregating populations and/or germplasm collections will greatly enhance the efficiency of developing high oleic peanut varieties with resistance to Sclerotinia blight.