To add better measurement and durability capabilities, the currently-used heat pulse probe (HPP) designs need to be improved using experimental and numerical sensitivity analyses. Only few experimental studies were conducted to examine the effect of HPP probe properties, calibration media, and operational procedures. However, such studies were traditionally focused on determining the heat properties and water content of the related medium. Recently, several studies are conducted to increase the HPP capabilities for determining of water fluxes but none of these studies were investigated experimentally with respect to the effects of HPP probe and its material properties on the sensitivity of detecting water and vapor fluxes. In this study, we will present experimental results of the effects of sensor locations and thermal properties of Delrin material used as HPP sensor body, the heater diameter, heating duration, and heat intensity on water and vapor flow in soils. Moreover, the experimental results will be validated with numerical studies using HYDRUS-2D/3D and COMSOL Multiphysics software.