Enhancing the nutritional value of food crops is a sensible strategy for improving human health and nutrition. Despite its importance, efforts to increase wheat grain protein content (GPC) and micronutrient levels have been hindered by their large environmental dependency and their complex inheritance. A potential source for nutritional improvement was detected in wild emmer wheat (Triticum turgidum ssp. dicoccoides), where a QTL for GPC (Gpc-B1) was shown to confer consistent GPC increases when introgressed into tetraploid and hexaploid wheat varieties (on average 14 g kg^-1 (~10-15%)). Recently, we showed that the high GPC allele also accelerates senescence (by 3-4 days) and increases Zn and Fe remobilization from leaves to developing grains (by 10-15%). Using a high-resolution genetic map based on ~9000 gametes, we delimited Gpc-B1 to a 7.4-kb region. This region included a single gene encoding a NAC transcription factor (NAM-B1). Modern cultivated wheat varieties carry a 1-bp insertion generating a frameshift mutation resulting in a non-functional transcription factor, whereas the ancestral wild emmer allele encodes a functional protein. To validate NAM-B1 as Gpc-B1, we reduced the RNA transcript levels of the multiple homologous copies in hexaploid wheat by RNA interference. Two transgenic events generated the expected phenotype: senescence was delayed by over 3 weeks and protein, Zn, and Fe grain content was decreased by over 30%. These results show that lines with higher amounts of NAM transcripts (non-transgenic controls) were able to remobilize more minerals, in a shorter time frame, therefore suggesting a more efficient remobilization to the grain. The potential impact of the introgression of the functional NAM-B1 allele in cultivated durum and bread wheat varieties will be discussed.